Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for ABI 7500 handbook

About this manual

Agilent Technologies presents the Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mixes, engineered to provide the fastest cycling times on the ABI 7500 Fast real-time PCR platform. These reagents allow researchers to complete experiments in as little as 29 minutes without compromising data quality.

What's inside

• Features
• Performance Benefits
• QRT-PCR Performance
• Sensitivity and Specificity
• Reliability Comparison

Frequently asked questions

What is the total run time for real-time experiments using these master mixes?

The new ultra-fast reagents allow the completion of real-time experiments in as little as 29 minutes.

What temperature is required for optimal performance during first strand synthesis with the one-step QRT-PCR reagents?

Optimal performance for first strand synthesis using a Moloney-based RT occurs at 50°C.

What is the total reaction volume used in the sensitivity and reliability tests described on page 2?

The reactions contained a total volume of 20 ul for both the sensitivity detection and reliability comparison tests.