This document is about the Brilliant III Ultra-Fast qPCR/QRT-PCR Master Mixes for ABI StepOnePlus Real-Time PCR System data sheet, introducing the features and characteristics of the product
Agilent Brilliant III Ultra-Fast qPCR and QRT-PCR Master Mixes are designed to provide the fastest cycling times on the ABI StepOnePlus real-time PCR instrument, completing experiments in under 40 minutes. These kits utilize a newly engineered Taq derived mutant and novel hot-start technology to minimize non-specific amplification and primer-dimer formation while maintaining high sensitivity and reproducibility.
| Cycling time | under 40 minutes |
|---|---|
| Compatible instruments | ABI StepOnePlus, ABI 7900HT, 7500 Fast |
| RT synthesis temperature | 50°C |
| Reaction volume assumption | 20 µl |
The new ultra-fast reagents allow the completion of real-time experiments in under 40 minutes.
Yes, the formulations are compatible with both sequence-specific probes and SYBR® Green dyes.
The efficiency is 95.7% with an RSq of 0.992.