This study describes the development of a robust high-throughput analytical method for the determination of 18 elements (15 trace elements and 3 electrolytes) in blood and serum samples using an Agilent Technologies 7500ce collision/reaction cell ICP-MS system. The only sample preparation necessary was dilution using an alkaline diluent containing ammonia, EDTA Triton X-100, and butan-1-ol. Instrument calibration was performed using external calibration with internal standardization. The performance of the method exceeded a previously-used magnetic sector HR-ICP-MS method by at least a factor of three in terms of sample throughput and matched the precision and detection limits of that method.
This document details the development of a robust, high-throughput analytical method for determining 18 elements (15 trace elements and 3 electrolytes) in blood and serum samples. The method utilizes an Agilent 7500ce collision/reaction cell (CRC) ICP-MS system and requires only a simple dilution of samples using a specific alka-line diluent containing ammonia, EDTA, Triton X-100, and butan-1-ol.
The study compares the performance of this CRC-ICP-MS method against a previously used magnetic sector HR-ICP-MS method. Results indicate that the new method exceeds the previous method in sample throughput by at least a factor of three while matching its precision and detection limits. The document provides specific instrument parameters, gas modes (hydrogen, helium, and standard), and analysis settings for the target analytes.
| Target Elements | Al, As, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Sb, Se, V, Zn, K, Mg, Na |
|---|---|
| Sample Dilution Factor | 20-fold |
| Internal Standard Concentration | 20 ng/mL |
| Total Acquisition Time | 208 s |
| Sample Throughput | up to 100 samples per batch |
| Detection Limit Target | 0.2 ng/mL for Co, Mo; 1.0 ng/mL for Ni |
The only sample preparation necessary was dilution using an alka-line diluent containing ammonia, EDTA Triton X-100, and butan-1-ol.
The Agilent 7500ce was used in three different gas modes: hydrogen, helium, and standard or no-gas mode.
The performance of the method exceeded a previously-used magnetic sector HR-ICP-MS method by at least a factor of three in terms of sample throughput.