The Agilent 7500c Inductively Coupled Plasma Mass Spectrometer (ICP-MS) was used for the fast, routine, and reliable analysis of 23 trace elements in urine.
The document details a method for analyzing 23 trace elements in urine samples using the Agilent 7500c Inductively Coupled Plasma Mass Spectrometer (ICP-MS) equipped with an Octopole Reaction System (ORS). The method involves a simple sample preparation step where urine is diluted 1/5 (v/v) with 1% nitric acid, allowing a single operator to prepare over 100 samples in less than an hour. The ORS collision/reaction cell, pressurized with helium or hydrogen, removes plasma and matrix-based interferences that typically inhibit trace analysis of elements like Chromium, Arsenic, and Selenium.
| Power | 1500 W |
|---|---|
| Outer gas flow | 14 L/min |
| Intermediate gas flow | 10 L/min |
| Nebulizer gas flow | 1.414 L/min |
| Torch injector tube diameter | 2.5 mm |
| Sample uptake rate | 0.4 mL/min |
| Integration time per mass | 1.0 s |
The urine samples were analyzed directly after a low dilution of 1/5 (v/v) with 1% nitric acid.
One operator can prepare more than 100 samples in less than 1 hour using this method.
Selenium was determined using hydrogen as a reaction gas to overcome interferences.