This document introduces the HPLC analysis of six different antibacterial drugs, including nalidixic acid, trimethoprim, sulfamethoxazole, chloramphenicol and furazolidone. The SB-C18 column provides excellent peak shape and selectivity for basic antibacterial drugs at low pH. The method is easy to use, reliable and accurate.
The document details a High-Performance Liquid Chromatography (HPLC) method for analyzing six specific antibacterial drugs: Nalidixic acid, Trimethoprim, Sulfamethoxazole, Furazolidone, Chloramphenicol, and another compound. The analysis utilizes an Agilent 1100 Series HPLC system equipped with a variable wavelength detector.
| Column | Zorbax SB-C18, 3.5 um, 4.6 x 75 mm |
|---|---|
| Guard Cartridges | SB-C18, 5 um, 4.6 x 12.5 mm |
| Mobile Phase A | 0.025 M KH2PO4 in water (pH = 3) |
| Mobile Phase B | acetonitrile |
| Flow rate | 1.0 mL/min |
| Temperature | 40 °C |
The analysis was performed using an Agilent 1100 Series HPLC with a variable wavelength detector.
The six drugs analyzed are Nalidixic acid, Trimethoprim, Sulfamethoxazole, Furazolidone, Chloramphenicol, and another unspecified compound.