This application note describes a rapid method for the simultaneous detection and quantification of 37 acylcarnitines and 12 amino acids in dried blood spots using the Agilent Triple Quadrupole LC/MS System in neutral loss scan, precursor ion scan and multiple reaction monitoring (MRM) data acquisition modes. With a run time of 1.6 minutes, the assay provides the ability to analyze 400-500 samples per day, and custom reports provide all the information needed to rapidly list the critical elements for the analysis.
This document details a clinical research method for the simultaneous analysis of 37 acylcarnitines and 12 amino acids in dried blood spots (DBS). The method utilizes the Agilent Triple Quadrupole LC/MS System with a run time of 1.6 minutes, allowing for the processing of 400-500 samples per day. The assay employs neutral loss scan, precursor ion scan, and multiple reaction monitoring (MRM) data acquisition modes to detect and quantify the analytes.
The procedure covers sample preparation involving extraction with methanol containing isotopic internal standards, filtration, derivatization with butanol-HCl, and reconstitution. The document provides specific instrument conditions for the Agilent 1200 Series LC system coupled with an Agilent 6460 Triple Quadrupole LC/MS, including mobile phases, flow rates, and mass spectrometer settings for positive ESI mode.
| Run time | 1.6 minutes |
|---|---|
| Samples per day | 400-500 |
| Acylcarnitines analyzed | 37 |
| Amino acids analyzed | 12 |
| Injection volume | 5 ul |
| Flow rate | 0.6 mL/min |
| Drying gas temperature | 300 °C |
| Capillary voltage | 4000 V |
The run time for the assay is 1.6 minutes.
The assay provides the ability to analyze 400-500 samples per day.
The method uses neutral loss scan, precursor ion scan, and multiple reaction monitoring (MRM) modes.