This article describes a method for separating trichlorobenzene from fish using Agilent EcoSpheres. EcoSpheres are a novel type of gel beads that have a unique structure and chemical composition that allows them to capture and separate organic contaminants. EcoSpheres have excellent performance and can effectively separate trichlorobenzene. The method is simple and easy to use and can be widely applied to the analysis of trichlorobenzene in environmental samples.
The document details a quality control procedure for separating low molecular weight contaminants, specifically trichlorobenzene, from high molecular weight lipid fractions in macerated sardine samples. The method utilizes gel permeation chromatography (GPC) with Agilent EcoSpheres media to purify the sample prior to analysis.
| Column Dimensions | 300 x 25 mm |
|---|---|
| Column Part Number | PL1310-0054 |
| Media Part Number | PL1460-4M03 |
| Eluent Composition | 50:50 mixture of cyclohexane and ethyl acetate |
| Flow Rate | 5 mL/min |
| Maximum Pressure | 300 psi (21 bar) |
| Maximum Flow Rate | 10 mL/min |
| Detection Wavelength | 254nm |
To separate low molecular weight contaminants from high molecular weight lipid fractions in macerated sardine samples.
A 50:50 mixture of cyclohexane and ethyl acetate.
Agilent Cirrus GPC software and Agilent PL DataStream.