This application note describes a method for using Agilent PL aquagel-OH MIXED 8 µm columns for size exclusion chromatography (SEC) analysis of polymers. The method can be used to determine the molecular weight distribution of polymers and to evaluate the performance of polymers under different conditions.
This document describes an application of Size Exclusion Chromatography (SEC) using Agilent Technologies equipment to analyze copolymers of vinylmethyl ether and maleic anhydride used in dental applications. The text details a comparison between two batches of a commercial copolymer, one successful and one failed, to illustrate the system's capability in quality control.
| Column dimensions | 300 x 7.5 mm |
|---|---|
| Column particle size | 8 um |
| Flow Rate | 1.0 mL/min |
| Eluent composition | 0.2 M NaNO3 + 0.01 M NaH2PO4 at pH7 |
| Detection method | Agilent PL-GPC 50 Plus (DRI) |
The analysis used an Accolumn set comprising 2 x Agilent PL aquagel-OH MIXED 8 um columns.
Column calibration was achieved using Agilent EasiVial PEG/PEO standards.
The standards cover a wide molecular weight range typically from 100 to 1,200,000 g/mol.