This document describes the method and results of well-to-well repeatability testing for a 96-well microplate using the Agilent Cary Eclipse Microplate reader. By adopting correct laboratory procedures and utilizing the reader's alignment function, variations in well-to-well volume and meniscus shape can be minimized. The Agilent Cary Eclipse Microplate reader has a well-to-well repeatability of less than 2%, compared to the 5% repeatability of conventional instruments.
This document describes the importance of well-to-well repeatability for obtaining high-precision results in microplate analysis. It highlights that the Agilent Cary Eclipse Microplate reader achieves repeatability of less than 2% for every single well in a 96-well plate, significantly better than conventional instruments.
| Well-to-well repeatability | less than 2% |
|---|---|
| Average %RSD | 1.75% |
| Excitation Wavelength | 490 nm |
| Emission Wavelength | 520 nm |
| PMT Voltage | 400 V |
The Agilent Cary Eclipse Microplate reader has repeatability for every single well in a 96 well plate of less than 2%.
The plate is aligned using the Auto-align procedure in the Cary Eclipse Align application.
Variation in well-to-well volume, variation in meniscus shape, and alignment of the microplate affect repeatability.