This application note illustrates the use of Agilent PL HFIPgel columns for the analysis of a polyamide sample.
This document details the analysis of polyamide and polyester materials using Gel Permeation Chromatography (GPC) with Hexafluoroisopropanol (HFIP) as a solvent. The text highlights that HFIP is a favored solvent for these polymers due to its low refractive index and ability to operate at near ambient temperatures, unlike alternative solvents like m-cresol.
| Columns | 2 x PL HFIPgel, 300 x 7.5 mm |
|---|---|
| Eluent | HFIP + 0.2 M NaTFAc |
| Flow rate | 1.0 mL/min |
| Injection volume | 200 pL |
| Temperature | 40°C |
| Detector | RI |
| System | Agilent PL-GPC 50 |
Hexafluoroisopropanol (HFIP) is used as the solvent.
HFIP is used at near ambient temperatures and has a low refractive index compared to polyesters and polyamides.
0.02 M NaTFAc is added to minimize aggregation of the polymer in solution.