This Application Note demonstrates the influence of system delay volume and system dispersion on the resulting resolution and sensitivity achieved for LC/MS experi- ments. The advantages of using a low delay volume LC system and optimized connec- tion of the LC to the MS are shown. The study illustrates that the Agilent 1290 Infinity LC system is an ideal partner for LC/MS instruments.
This document investigates the impact of system delay volume and tubing dispersion on the performance of Liquid Chromatography-Mass Spectrometry (LC/MS) systems. It highlights that lower delay volumes and optimized connections are critical for maintaining high resolution and sensitivity, particularly for early-eluting compounds.
The study utilizes an Agilent 1290 Infinity LC system to compare separation results under standard low-delay conditions versus extended delay configurations. Additionally, it analyzes the effects of varying capillary inner diameters connecting the LC column outlet to the mass spectrometer source, demonstrating how larger diameters significantly degrade chromatographic resolution.
The flow rate was set to 0.5 mL/min.
The column temperature was maintained at 40°C.