Sample Clean-up by Gel Permeation Chromatography Using Agilent EcoSpheres
Governments require the analysis of environmental and food samples for contaminants, but complex biological matrices often contain interfering compounds like lipids and proteins. Gel permeation chromatography (GPC) is used to isolate these contaminants by removing such high molecular weight interferences before further analysis.
The document details a method using Agilent EcoSpheres, an economic loose microporous material in dry-powder form. The text provides specific conditions for a test separation of an EPA test mix containing corn oil, bis(2-ethylhexyl) phthalate, methoxychlor, perylene, and sulfur, demonstrating the suitability of EcoSpheres for applications covered by EPA 3640A.
It is a loose microporous material used to isolate contaminants from high molecular weight interferences in complex biological matrices.
The specified column is 450 x 25 mm.
The system requires a flow rate of 5 mL/min.