The document describes the use of Agilent EnviroPrep in single-stage extract clean-up for the determination of pesticides using high performance gel permeation chromatography (GPC). This method can be used for the determination of pesticides in various organic matrices, such as soil and animal tissue. GPC easily eliminates higher molecular weight fractions such as lipids, polymers, natural resins, and dispersed high molecular weight components. These species are relatively non-volatile, so their removal from the extract before GC/MS analysis of pesticides and other pollutants extends the lifetime of the GC column and improves chromatographic efficiency. Agilent EnviroPrep GPC columns, with their small pore size (100Å) and low exclusion limit (4,000 g/mol), are ideal for preparative applications, offering high resolution and high loading (>10 mg on-column loading of the components in this analysis).
High performance gel permeation chromatography (GPC) provides a simple, one stage clean-up procedure for the determination of pesticides in organic matrices such as soil and animal tissue. This process eliminates higher molecular weight fractions like lipids and polymers to extend GC column lifetime and improve efficiency.
The document details an application using Agilent EnviroPrep GPC columns with small pore sizes (100A) and low exclusion limits (4,000 g/mol). These columns offer high resolution and high loading capabilities. A test separation of a five-component mixture demonstrates the ability to resolve relatively high molecular weight components from lower molecular weight compounds of interest.
The columns support high loading of greater than 10 mg.
The columns can be operated up to 2,200 psi.
The columns can be used with eluent flows of up to 10 mL/min.