This Application Note describes a method for the quantifi cation of two antibacterial drugs, olaquindox and carbadox in animal feedstuff using the Agilent 1260 Infinity Binary LC system.
This document presents a simple and sensitive analytical method for the routine monitoring of banned substances, specifically the growth promoters olaquindox and carbadox, in animal feedstuff. The method utilizes the Agilent 1260 Infinity Binary LC system equipped with a Max-Light 60 mm high-sensitivity flow cell and UV detection at specific wavelengths (375 nm for olaquindox and 306 nm for carbadox). The system configuration includes the G1312B Binary Pump, G1379B Degasser, G1367E Autosampler, G1330B Thermostat, and G1316B Column Compartment, controlled by ChemStation B.04.03 software.
| Column | Agilent ZORBAX SB-Aq 3.0 x 100 mm, 3.5 ym |
|---|---|
| Flow rate | 0.75 mL/min |
| Detection wavelength for olaquindox | 375 nm |
| Lower limit of quantification | 50 ng/mL |
| Software | ChemStation B.04.03 |
The lower limit of quantification achieved for both molecules was 50 ng/mL.
The area response was found to be linear up to 5,000 ng/mL.