This document describes the method of using the Agilent 1260 Infinity Analytical SFC system for fast and ultra-fast SFC analysis. By using high flow rates, sub-2-µm particle technology, and short columns, separation times of less than 30 seconds with good resolution can be achieved. This method can increase sample throughput and reduce cost of ownership.
The document describes how to achieve separation times of less than 30 seconds for drug development applications. This is accomplished by utilizing high flow rates, sub-2-micron particle technology, and short columns within the Agilent Analytical SFC system.
The specific setup includes the Aurora Fusion AS, Agilent 1260 Infinity SFC Binary Pump, High Performance Autosampler, Thermostatted Column Compartment, Diode Array Detector VL, Vacuum Degasser, and a ZORBAX RX-Sil column. The results show high separation efficiency with over 9000 plates per peak in ultra-fast analyses.
The system separated the mixture in less than 30 seconds.
The total gradient time is 14 seconds.