This application note describes an effi cient and economical RTL-GC/FID method for determination of fatty acids in infant formulas. The fatty acids were converted to FAMEs using acetyl chloride-methanol methyl esterifi cation method. Agilent J&W HP-88 capillary GC column provides excellent separation for complex FAMEs including cis-trans isomer separation.
This document details an analytical method for determining fatty acids in infant formulas by converting them to fatty acid methyl esters (FAMEs). The method utilizes the Agilent J&W HP-88 capillary GC column to achieve excellent separation of complex FAME mixtures, including trans-isomers.
The note provides specific experimental procedures for sample preparation and lists the instrumental conditions for both GC/FID and GC/MSD systems. It includes a comprehensive table of 37 FAMEs with their retention times, CAS numbers, and molecular weights, demonstrating the column's performance on a 37-component mixture.
| Column Length | 100 m |
|---|---|
| Column Inner Diameter | 0.25 mm |
| Film Thickness | 0.2 µm |
| Part Number | 112-8847 |
| GC Inlet Temperature | 260 °C |
| Split Ratio | 30:1 |
| Carrier Gas (FID) | Nitrogen |
| Carrier Gas (MSD) | Helium |
| Oven Start Temperature | 140 °C |
| Oven End Temperature | 240 °C |
The document describes an efficient RTL-GC/FID method where fatty acids are converted to FAMEs using acetyl chloride-methanol methyl esterification.
The analysis was performed on an Agilent 7890A GC-FID system and an Agilent 7890 GC-5975C MSD system.