This file describes the chiral separation and enantiomeric excess (EE) determination of three model chiral compounds using Supercritical Fluid Chromatography using the Agilent 1260 Infinity Analytical SFC system. The low detector noise of the system due to the SFC pump technology resulted in excellent sensitivity for trace analyte detec- tion of chiral impurities. When applying method optimization sufficient resolution was achieved for all compounds even under mass overload conditions. This allowed for the quantitation of impurities at 0.05 to 0.1% levels.
The document describes a study on chiral separation and enantiomeric excess (EE) determination performed by Supercritical Fluid Chromatography (SFC). It utilizes the Agilent 1260 Infinity Analytical SFC system, which combines an LC module with an Aurora SFC Fusion A5 for CO2 conditioning. The text highlights that the system's low detector noise allows for excellent sensitivity in detecting trace chiral impurities at levels as low as 0.05% to 0.1%. Three model compounds—N-(3,5-dinitrobenzoyl)leucine, thalidomide, and 1,1'-bi-2-naphthol—were analyzed using a generic chiral SFC method.
The system is used for chiral separation and enantiomeric excess determination of chiral compounds.
The system allows for the quantitation of impurities at 0.05 to 0.1% levels.
The system enables the determination of enantiomeric excess at impurity levels below 0.05%.