This application note describes an improved microextraction method for sample preparation that can be used to analyze a variety of therapeutic categories of pharmaceuticals on an Agilent Poroshell 120 column. The method is simple, fast, economical, and effective for therapeutic drug monitoring and forensic chemistry.
This document presents an analytical method for determining various pharmaceuticals in whole blood samples. The method utilizes a modified mini-extraction procedure based on the QuEChERS approach, involving protein precipitation and dispersive solid-phase extraction (d-SPE) for cleanup. The study evaluates different extraction solvents and salt buffers to optimize sample preparation for complex biological matrices.
| Column dimensions | 2.1 x 100 mm |
|---|---|
| Particle size | 2.7 um |
| Flow rate | 0.4 mL/min |
| Column temperature | 30 °C |
| Centrifuge speed | 18,000 rpm |
| d-SPE tube capacity | 2 mL |
The AOAC buffered salts yielded the cleanest extract, visually, and were chosen for use with the d-SPE.
Analytes are isolated from spiked samples with recoveries above 80% on average.
The RSDs are typically below 10% for a wide range of substances.
The method delivers limits of detection below 10 ng/mL.