This document introduces orthogonality in reversed-phase liquid chromatography and achieves high resolution by comparing the selectivities of different Agilent Polaris and Agilent Pursuit columns.
Orthogonality in chromatography refers to alternative selectivity between separations, which is essential for addressing insufficient resolution that can mask impurities or degradation peaks. This document details a study comparing different Agilent Polaris and Pursuit stationary phases to identify pairs of columns with orthogonal retention patterns.
The research screened ten premier Polaris and Pursuit columns against a broad range of analytes under identical conditions using an Agilent ProStar HPLC System. By analyzing the scatter in retention factors, the authors identified specific column combinations that exhibit high orthogonality, helping end-users save time and effort during method development.
Orthogonality refers to alternative selectivity between separations, achieved by modifying method parameters or choosing stationary phases with different selectivities.
Orthogonal or 2D separations are needed to address insufficient resolution that can mask an impurity or a sample degradation peak.
The analysis was performed at a flow rate of 1.0 mL/min.
UV Detection used for most analytes was 254 nm.