Describes the use of Mycoplasma Plus PCR Primer Set
This document provides instructions for using the Mycoplasma Plus PCR Primer Set, Catalog #302008-12, a research-use-only kit designed to detect Mycoplasma infections in cell cultures. The set includes PCR primers, internal control templates, positive control templates, and StrataClean resin.
| Final reaction volume | 50 μl |
|---|---|
| Detection limit (supernatant) | 100 μl of cell culture supernatant |
| Amplification product size | 874-bp |
No, the Mycoplasma Plus PCR primer set is for research use only and is not intended for clinical diagnosis.
Set a thermal cycler heat block at 95°C or prepare a boiling water bath.
For optimal accuracy, use Taq2000 DNA polymerase.