Agilent DNA 1000 Kit Guide is a product manual published by Agilent Technologies, Inc.
The document serves as a user manual for the Agilent DNA 1000 Kit, detailing the procedures required to perform DNA analysis. It covers the necessary equipment, including the Chip Priming Station and Vortex Mixer, alongside the Agilent 2100 Bioanalyzer.
The guide outlines specific protocols for preparing reagents such as the Gel-Dye Mix, Marker, Ladder, and Samples. It further instructs users on setting up the assay equipment, loading the chip, running the analysis via Expert software, cleaning electrodes, and interpreting the resulting electropherograms.
| Sizing range | 25-1000 bp |
|---|---|
| Typical sizing resolution (25-100 bp) | + 5 bp |
| Sizing accuracy (100-500 bp) | + 5% |
| Kit stability | 4 months |
| Quantitative range | 0.1-50 ng/ul |
The sizing range covers DNA fragments from 25 to 1000 base pairs (bp).
The quantitative range is from 0.1 to 50 ng/ul.