Agilent Technologies pFB and pFB-Neo Retroviral Vectors Instruction Manual

About this manual

This document serves as an instruction manual for the Agilent Technologies pFB and pFB-Neo Retroviral Vectors (Catalog #217563 and #217561). It details the materials provided with each kit, including specific quantities of vectors and control plasmids. The guide outlines storage conditions at -20°C and includes a notice regarding patent coverage for the translation enhancer in the pFB-Neo vector.

What's inside

• Materials Provided
• Storage Conditions
• Introduction
• The pFB Vector
• The pFB-Neo Vector
• pFB-Luciferase Control Vector
• Replication-Defective Retroviral Gene Transfer System
• Biosafety Considerations
• Ligation of Vector and Insert
• Recombination and Host Strains

Specifications

Frequently asked questions

What is the recommended storage condition for these vectors?

All components should be stored at -20°C.

Which patent covers the translation enhancer of the pFB-Neo vector?

The use is covered by U.S. Patent No. 4,937,190.

What biosafety level are retroviral vectors designated as?

They are designated as Biosafety Level 2.