Transpack Packaging Extract For Lambda Transgenic Shuttle Vector Recovery instruction manual
The document serves as an instruction manual for the Transpack Packaging Extract, a reagent used in the Big Blue transgenic rodent mutagenesis assay system. It details the recovery of a recoverable transgenic lambda phage shuttle vector from genomic DNA isolated from rodent tissues. The extract rescues the shuttle vector and packages it into viable lambda phage particles capable of infecting Escherichia coli.
| Catalog Numbers | #200220, #200221, #200223 |
|---|---|
| Storage Temperature | -80°C |
| Host Strain | Escherichia coli strain SCS-8 |
All components must be stored at -80°C, and the extract should not be thawed prior to use.
No, the product is extremely temperature sensitive and cannot be refrozen once thawed.
Genomic DNA with high molecular weight fragments greater than 100 kb gives the best results.