Agilent pBK-CMV Phagemid Vector Instruction Manual

This document is an instruction manual for the pBK-CMV Phagemid Vector, which provides information on the usage, storage conditions, technical services, and product warranty of the vector.


Agilent pBK-CMV Phagemid Vector Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
32
File format
PDF
File size
358 KB
Published
06 June, 2012
Updated
29 September, 2023
MD5 checksum
719CB1972347A1DCA35D31441DCA11FA

About this manual

This document is an instruction manual for the pBK-CMV phagemid vector (Catalog #212209) manufactured by Agilent Technologies. The vector allows for expression in both eukaryotic and prokaryotic systems, driven by a cytomegalovirus promoter for eukaryotes and a lac promoter for bacteria.

What's inside

  • Materials Provided
  • Storage Conditions
  • Transformation with the pBK-CMV Phagemid Vector
  • Blue-White Color Selection
  • Eukaryotic Screening
  • Prokaryotic Expression Screening Protocol
  • Transcription by T3 and T7 RNA Polymerase
  • Site-Directed Mutagenesis

Specifications

Vector TypepBK-CMV Phagemid Vector
Eukaryotic PromoterCytomegalovirus (CMV) immediate early promoter
Resistance GenesNeomycin and Kanamycin resistance

Frequently asked questions

What are the storage conditions for the pBK-CMV Phagemid Vector?

The pBK-CMV Phagemid Vector should be stored at -20°C.

What is the storage condition for the Helper Phage?

The Helper Phage (R408) must be stored at -80°C.

How are colonies containing vector without insert identified?

Colonies containing the vector without an insert will appear blue in the presence of X-gal and IPTG.

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