The QuikChange Lightning Multi Site-Directed Mutagenesis Kit is a reagent kit for site-directed mutagenesis, which is fast, efficient, and flexible. The kit provides a simple three-step protocol for constructing various engineered mutants. The limited product warranty ensures the quality of the product and technical support is available.
The document provides instructions for the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Catalog #210514 and #210516). It outlines the three-step protocol involving mutant strand synthesis, Dpn I digestion, and transformation of XL10-Gold Ultracompetent Cells. The manual lists all materials provided in the kit and additional reagents required for color screening.
| Catalog Numbers | #210514 and #210516 |
|---|---|
| Reactions for Catalog #210514 | 30 total reactions (includes 2 control) |
| Enzyme Blend Quantity (#210514) | 30 reactions |
| Buffer Volume per Kit | 200 ul |
XL10-Gold Ultracompetent Cells and pUC18 Control Plasmid must be stored at -80°C.
All other components should be stored at -20°C.
The protocol requires 14-ml BD Falcon polypropylene round-bottom tubes.