This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra-Fast QPCR Master Mix with the 7500 Fast Real-Time PCR System from Applied Biosystems.
This document serves as a Quick Reference Guide for preparing and running reactions using the Stratagene Brilliant III Ultra-Fast QPCR Master Mix on the ABI 7500 Fast Real-Time PCR System. It details the steps to prepare reagent mixtures, including diluting the reference dye and combining master mix components with experimental DNA templates such as genomic DNA or cDNA.
The protocol requires adding experimental DNA to bring the final reaction volume to exactly 20 μl.
The guide suggests using between 5 pg and 100 ng of genomic DNA for each reaction.
The guide suggests using between 0.1 pg and 100 ng of cDNA for each reaction.