This document describes the features and characteristics of Agilent Technologies' SureSelectXT Target Enrichment System for Illumina Paired-End Sequencing Library Illumina HiSeq and MiSeq Multiplexed Sequencing Platforms. The product is manufactured with Agilent SurePrint Technology and is intended for research use only, not for use in diagnostic procedures.
This document describes a protocol specifically developed and optimized for biotinylated RNA oligomer libraries to enrich targeted regions of the genome. It is designed for use with Illumina paired-end multiplexed library preparation on HiSeq and MiSeq sequencing systems.
The guide details the complete workflow, including sample preparation (shearing, end repair, ligation), hybridization steps using SureSelect capture libraries, post-hybridization amplification to add index tags, and final quality assessment. It includes procedural notes, safety information, required reagents and equipment lists, and alternative equipment combinations.
| Supported Sequencing Platforms | Illumina HiSeq and MiSeq |
|---|---|
| Technology Basis | Biotinylated RNA oligomer libraries |
| Document Version | 1.3.1 |
The system uses biotinylated RNA oligomer libraries to enrich targeted regions of the genome.
No, this product is for research use only and not for use in diagnostic procedures.