This document provides the instruction manual and ordering information for Agilent's Brilliant III Ultra-Fast QPCR Master Mix. The product is a ultra-fast real-time quantitative PCR kit designed for research use only. It offers high sensitivity, stability, and reliability for fast and accurate qPCR experiments.
The document provides instructions for using the Brilliant III Ultra-Fast QPCR Master Mix, a single-tube reagent designed for accelerated quantitative PCR amplifications with fluorescent TaqMan probes. It is compatible with instruments such as the ABI StepOnePlus and Bio-Rad CFX96 systems.
| Catalog Numbers | #600880 (single kit), #600881 (10-pack kit) |
|---|---|
| Reaction Volume Capacity | 400, 20-μl reactions |
| Magnesium Chloride Concentration (1×) | 5.5 mM |
| Reference Dye Excitation Wavelength | 584 nm |
| Reference Dye Emission Wavelength | 612 nm |
It is designed for use on the ABI StepOnePlus and Bio-Rad CFX96 real-time PCR instruments, as well as other fast-cycling systems like the ABI 7900HT and 7500 Fast.
The optimal concentration can be determined by varying the final concentration from 150 to 600 nM.
For a StepOnePlus or 7900HT Fast instrument, dilute the dye 1:50 to achieve a final concentration of 300 nM in the reactions.