Agilent SureSelect RNA Target Enrichment Protocol Version 2.2.1 User Guide

About this manual

This document describes recommended operational procedures to capture transcripts of interest using the Agilent SureSelect RNA Capture Kit and sample preparation kits. The protocol is specifically developed and optimized to use Biotinylated RNA oligomer libraries, or Bait, to enrich targeted regions of the transcriptome for next-generation sequencing.

What's inside

• Before You Begin
• Sample Preparation
• Hybridization
• Addition of Index Tags by Post-Hybridization Amplification
• Reference

Frequently asked questions

What is the intended use of this protocol?

This guide describes procedures to capture transcripts of interest using the Agilent SureSelect RNA Capture Kit and sample preparation kits for next-generation sequencing.

What specific platform does this protocol use?

This guide uses the Illumina paired-end multiplex sequencing platform for library preparation.

How should frozen reagent stock solutions be thawed?

Thaw the aliquot as rapidly as possible without heating above room temperature.