Agilent Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Instruction Manual

This is an instruction manual for Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix product, which introduces the product's features and usage


Agilent Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
14
File format
PDF
File size
339 KB
Published
06 June, 2012
Updated
01 October, 2023
MD5 checksum
AA3483BAD64CB064A14E58DA33E660BD

About this manual

This document is an instruction manual for the Agilent Technologies Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Catalog #600882/#600883). The kit includes a 2x master mix containing a mutated Tag DNA polymerase, dNTPs, magnesium chloride, and SYBR Green I dye, along with a passive reference dye. It is designed for accelerated quantitative PCR amplifications on instruments such as the ABI StepOnePlus, Bio-Rad CFX96, ABI 7900HT, and 7500 Fast systems.

Specifications

Reactions per single kit400, 20-µL reactions
Storage temperature (initial)-20°C
Storage temperature (after thawing)4°C for up to three months or -20°C
Compatible instrumentsABI StepOnePlus and Bio-Rad CFX96 real-time PCR instruments

Frequently asked questions

What additional materials are required to use this master mix?

A spectrofluorometric thermal cycler and nuclease-free PCR-grade water are required.

What is the recommended storage condition for the master mix?

Store at -20°C upon receipt. After thawing, it can be stored at 4°C for up to three months.

Is this product suitable for diagnostic procedures?

No, it is for research use only and not for use in diagnostic procedures.

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