Key facts
Start here
TOYOBO QPK-201/QPK-201T SYBR Green Realtime PCR Master Mix User Manual
The SYBR® Green Realtime PCR Master Mix (code QPK-201, QPK-201T) is a 2× concentrated master mix developed on Taq DNA polymerase. It contains all components needed for SYBR Green I detection except primers and sample DNA, and is compatible with most real‑time PCR instruments such as Roche LightCycler™ and Applied Biosystems ABI PRISM®.
The manual includes sections on product composition, required additional reagents and equipment, detailed preparation and cycling protocols, a troubleshooting FAQ, and a list of related products. Storage, handling, and safety notes are also provided. This manual is written in Chinese.
TOYOBO QPK-201 specifications
| Formulation | 2× master mix |
|---|---|
| Fluorescent Dye | SYBR® Green I |
| Additive | BSA |
| Reference | Passive reference |
| Enzyme Inhibitor | Anti‑Taq monoclonal antibody |
| Storage (long term) | -20 °C, light‑protected |
Source: TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual
All TOYOBO QPK-201 documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
Why might the amplification curve be messy?
It can be caused by PCR instrument settings or by problems with the reagents.
From: TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual
Why can quantitative values show poor reproducibility?
Both instrument configuration and reagent quality can affect reproducibility.
From: TOYOBO QPK-201 Realtime PCR Master Mix SYBR Green User Manual
How many reactions can be performed with the QPK-201 and QPK-201T kits?
QPK-201 provides 200 reactions (50 µL each) and QPK-201T provides 40 reactions (50 µL each).
From: TOYOBO QPK-201/QPK-201T SYBR Green Realtime PCR Master Mix User Manual