Industrial

Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase. Note selectivity and resolution differences for peptides S1 and S2. Peptides S1-S5 are decapeptides that differ in amino acids at two locations, causing only slight differences in hydrophobicity.

This document describes how to improve separation by taking advantage of different bonded-phase chemistries. When using StableBond sterically protected phases, both long-chain and short-chain bonded phases are stable.

This document introduces the effect of column aging with acidic mobile phase on ZORBAX 300SB-C8 (4.6 x 150 mm) (Agilent P/N: 883995-906) column.

This document introduces the separation results of Agilent Technologies' SB-C18, SB-C8, SB-C3, and SB-CN columns at 0-70% B in 36 minutes, and compares them with the separation results on 300Å StableBond columns.

Using elevated temperatures for the reverse-phase separation of peptides and proteins can increase column efficiency, change selectivity, and improve peak shape.

The document discusses the effect of bonded-phase on the resolution and retention of polypeptides. It highlights that differences in bonded-phase selectivity can significantly improve the resolution in reversed-phase analysis of peptides and proteins. The separation between peaks 2 and 3, peaks 5 and 6, and peaks 8 and 9 illustrate these selectivity changes. Additionally, it mentions that temperature can also be used to achieve desired separations. The steric protection of ZORBAX SB packings makes them well suited for chromatography at very low pH.

This document describes how to use small sample injection volumes in size exclusion chromatography

This experiment report introduces the effect of column length on efficiency and resolution. When appropriate, two or more SEC columns may be connected in series to achieve a desired separation.

This document investigates the effect of mobile-phase ionic strength on neutral and strongly basic antibodies. The results show that strongly basic molecules interact with silica-based column packings to a much greater extent than neutral molecules. Additionally, the concentration of phosphate buffer has an impact on peak shape and retention time.

The document introduces a high-resolution chiral separation technology for Fluoxetine (Prozac) enantiomers using the ULTRON ES-OVM column

This document introduces a method for separating immunoglobulins using size-exclusion chromatography. The method uses a high-performance ZORBAX particle that is extremely rugged, even under high back-pressures. This characteristic allows higher flow rates and shorter run times for high-throughput experimentation. The method can also reduce the injection volume of an antibody sample without loss of sensitivity, when using smaller-diameter columns.