Agilent Manuals

Agilent Multimode Inlet is a gas chromatography inlet that can introduce a large amount of sample (5 µL to 250 µL or more) into Agilent 7890 Series GC systems, thereby reducing detection limits while maintaining high sample throughput and quality of results.

High-sensitivity detection cell for the Agilent Capillary Electrophoresis system represents a major advance in detection technology for capillary electrophoresis, increasing sensitivity by an order of magnitude.

cIEF is a method used to run on the Agilent Capillary Electrophoresis (CE) system. It can separate proteins and peptides according to differences in their isoelectric points (pI). The pI of a protein is an important characteristic and cIEF can be used to either determine a protein's pI or to perform separations based on pI differences. Separations based on pI are used in protein analysis for identification, purity assessment, degradation checking, microheterogeneity analysis, clinical diagnosis, and the analysis of complex protein mixtures. The cIEF method described here is a two step method. First, the capillary is filled with a mixture of protein sample and ampholytes which is then focused by applying a high field strength (V/cm). The ampholytes form a pH gradient in the capillary and the proteins are focused into sharp zones at the pH region which corresponds to their pI. In the second step the contents of the capillary are mobilized past the detector by apply- ing pressure to the inlet vial while maintaining the field strength. Focused peaks are monitored by the detector and appear in order from basic to acidic proteins. TEMED is used as a spacer between the outlet end of the capillary and the detec- tion point so that focusing of the proteins takes place between the capillary inlet and detectio

This note provides recommendations for the use of extremely short effective length capillaries in CE. It discusses the advantages of using very short capillaries (less than 10 cm) and the limitations that must be considered when using extremely short capillaries in a CE instrument. The note also introduces methods of injecting samples into extremely short capillaries, including injection from the outlet end of the capillary and injection from the inlet end of the capillary.

This document introduces the working principle of Agilent 1200 Series Evaporative Light Scattering Detector (ELSD) and provides a method for using mobile phase compensation to significantly improve the quantification of unknowns.

This document introduces the integration of MSD Productivity ChemStation with OpenLAB ECM, including the functions of ECM, the advantages of integration, and how to use it

Ultra high performance liquid chromatography (UHPLC) columns with sub-2-μm particles offer high productivity and resolving power. Shorter columns with these small particles allow analysts to achieve faster runtimes while maintaining column efficiency and resolution. Longer columns with these small particles enable additional resolution for complex mixtures. However, smaller particles produce higher backpressure. When the backpressure is too high, the column will not run. This document introduces the Agilent ZORBAX Rapid Resolution High Definition (RRHD) columns, which are designed to operate at up to 1200 bar of pressure. In this high-pressure domain, analysts can achieve even more speed and resolution for complex separations.

This document compares the Agilent 6540 UHD Q-TOF and Thermo LTQ Orbitrap XL for fast UHPLC applications and concludes that the Agilent 6540 UHD Q-TOF provides superior resolution. The system offers up to ten-fold better resolving power than the Thermo LTQ Orbitrap XL and is suitable for laboratories requiring improved sample analysis speed and accurate mass analysis.

The Agilent 6540 UHD Q-TOF produces more accurate isotope ratios than Thermo LTQ Orbitrap XL

This document describes a comparison between the Agilent 6540 UHD Q-TOF and Thermo LTQ Orbitrap XL mass spectrometers. The Agilent 6540 UHD Q-TOF demonstrates better detection capability with a higher in-spectrum dynamic range compared to the Thermo LTQ Orbitrap XL.

Agilent 1290 Infinity Binary Pump is an ultra-high pressure liquid chromatography pump with dual columns that can deliver a flow rate of 0.05-5 mL/min at pressures up to 1200 bar.

This document introduces the performance characteristics of the Agilent 1290 Infinity Thermostatted Column Compartment. The product has new QuickChange valves, two heated zones and a 100°C operating temperature, making it more convenient to use.

This article introduces the High Matrix Introduction system for Agilent 7500ce ICP-MS, which allows the analysis of soil samples without the need for dilution and with slightly improved method detection limits. This system improves productivity and reduces costs.

This document introduces the Performance Characteristics of the Agilent High Matrix Sample Introduction (HMI) Accessory for the 7500 Series ICP-MS Product Overview, which can improve the performance of ICP-MS in high-concentration samples and reduce dissolved solids (TDS) levels to achieve better and more stable performance.