This article introduces a new hybridization apparatus and method that improves the hybridization quality, consistency, and ease of use of the existing system. The apparatus improves hybridization quality by providing a hybridization chamber made from inert materials that enable easy hybridization buffer mixing. Consistency is improved through material quality and high-quality manufacturing. Finally, ease-of-use is enhanced through protocols that utilize standard lab equipment and a straightforward hybridization chamber assembly. The apparatus has been validated for use with Agilent's in-situ synthesized oligonucleotide microarrays and exhibits equivalent or superior experimental performance compared to Agilent's current standard hybridization method.
This document introduces a new hybridization apparatus designed to improve the quality, consistency, and ease of use for hybridizing in-situ synthesized oligonucleotide microarrays. The new system replaces legacy multi-piece chambers with a simplified design comprising four interlocking metal pieces and a glass backing slide.
| Microarray Format | 1" x 3" microscope slide |
|---|---|
| Total Features | 10,807 |
| Probe Type | 60-mer yeast probes |
| Assembly Time | ~1.5 min |
| Disassembly Time | ~15 sec |
| Signal Increase | 36% average |
| Signal CV% Reduction | 14-16% to 6-7% |
The new hybridization chamber is model G2534A.
It reduces the time required to assemble, load, and disassemble the chamber compared to legacy models.
Yes, it achieves an increase of 34-38% in background subtracted signal.