Agilent Manuals

This document describes a high-temperature simulated distillation method for analyzing heavy crude oils and petroleum blends. The method uses a new metal column that has been deactivated by a proprietary process to provide excellent performance and exceptional durability at high temperatures.

The Ultra Inert liners with wool provide superior inertness for accurate analysis of semivolatile analytes by GC/FID, homogeneous sample mixing and evaporation, and maximum column and detector protection.

This document presents the preliminary results of a collection on an unusual macromolecular RNA G-quadruplex structure. The single crystal data was collected to 2.2Å resolution, providing us with a detailed view of the RNA’s sugar pucker and hydration structure. The efficient data collection geometry and associated high redundancy of data has enabled us to also visualize the anomalous scattering from the ions in the central channel, and confirm the location of the bromine atoms included to assist in phasing.

This document describes the analysis of drugs of abuse using GC/MS with the Ultra Inert Inlet Liners with Wool. These liners provide excellent inertness, homogeneous sample mixing and evaporation, non-volatile residue trapping, and column and detector protection for drugs of abuse screening.

This application note describes a rapid and sensitive LC/MS/MS method for the detection and analysis of four aflatoxins (B1, B2, G1, and G2) that provides detection down to 1 ppb. The 500 Ion Trap MS/MS capabilities result in the collection of clear, baseline resolved chromatographic peaks for target compound quantitation.

This document describes a method for the rapid, sensitive and reproducible detection of seven common drugs of abuse using the Agilent 500 Ion Trap LC/MS.

This note describes the analysis of albumin proteins using Agilent ProSEC 300S columns. Size exclusion chromatography (SEC) is used to investigate the aggregation of albumin proteins in solution, including dimers and trimers. This technique is of interest in biomedical applications.

This document introduces the key technology in protein analysis-size exclusion chromatography (SEC), and illustrates the principle, characteristics and application of SEC with Agilent ProSEC 300S column as an example

Due to the current global acetonitrile shortage, Agilent Technologies has been investigating a range of alternative solvents that could be used for reversed phase HPLC analysis of peptides, which maintain the efficiency and selectivity that a PLRP-S 100Å 10 µm gives for this application when used with acetonitrile. This report details how a 385-LC ELS detector can be used to prevent the increased background absorbance of the mobile phase eluents when run on a UV detector. The 385-LC ELSD is capable of evaporating a wide range of different solvents, resulting in completely flat baselines and sharp peaks, even during gradient elution.

This document investigates the structure of clindamycin using mass spectrometry and nuclear magnetic resonance spectroscopy. The data allows for the rapid confirmation of the chemical structure.

This application note describes a liquid separation method using Agilent Bond Elut Plexa PCX for mixed mode ion exchange to fractionate acidic, neutral, and basic drugs from plasma. The method uses mixed mode polymer ion exchange technology to retain neutral and acidic compounds from biofluids via hydrophobic interactions and concentrates basic compounds by interacting with the sorbent’s cation exchange properties.

This document presents a case study on the application of Agilent's 610 FTIR Fourier Transform Infrared (FTIR) microscopes for material analysis. It highlights the ability of the microscopes to characterize the spatial distribution of components and identify the chemical nature of a sample. The document also discusses the various measurement modes and the added capability of automated infrared mapping analysis with a motorized sample stage.