Agilent Manuals

The document introduces a method for the determination of 5-MOP and 8-MOP in lemon oil and orange oil using the Agilent 1290 Infinity LC system. At 1000 bar, the total analysis time in reversed-phase (RP) liquid chromatography (LC) with acetonitrile and water as mobile phase components could be reduced to 4 min. In the framework of green chromatography, and the present ace- tonitrile shortage, mobile phases constituted of water/methanol and water/ethanol were compared to water/acetonitrile. The influence of the nature of organic modifiers on selectivity and peak capacity was investigated. The figures of merit of the different methods were compared for the determination of 5-MOP in a lemon oil sample con- taining approximately 50 mg/kg. 8-MOP was not detected at the ppm level. The detection limit for both furocoumarins was approximately 1 mg/kg citrus oil.

This application note describes a rapid method for screening and confirmation of melamine, ammelide, ammeline and cyanuric acid in infant formula and soy meal using the Agilent 7890A/7000A Series Triple Quadrupole GC/MS and backflushing with a Purged Ultimate Union. The extraction and derivatization procedures are the same as those used in the FDA GC/MS method. Excellent linearity (R2 > 0.99) was obtained in the range of 0.16 to 2.5 ppm, with run times less than 15 minutes.

This article describes the analysis of saponins in spine date seed using Agilent ZORBAX Solvent Saver HT (1.8 µm) columns. The two main active compounds, Jujuboside A and B, were extracted and analyzed by high performance liquid chromatography (HPLC) with Agilent ZORBAX Eclipse Plus C18 LC columns. The method was first developed on a traditional 4.6 mm × 150 mm, 5 µm column and then transferred to a ZORBAX Eclipse Plus Solvent Saver High Throughput (1.8 µm) C18 column, 3.0 mm × 50 mm, 1.8 µm. The advantages of the Solvent Saver HT column include low solvent consumption and faster analysis. An evaporative light scattering detector (ELSD) was used due to the lack of chromophores in the two compounds. This provided higher sensitivity and a cleaner baseline compared with UV detection.

The method is used for the simultaneous determination of four β2-agonist residues of terbutaline, salbutamol, clenbuterol and formoterol in pork. The method uses Agilent SampliQ SCX solid-phase extraction (SPE) cartridges and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to measure the residues of four β2-agonists in pork. The method provides a sub-ng/g level of limit of detection (LOD) for all four β2-agonists in pork. The dynamic calibration ranges for these compounds are obtained from 0.25 to 5 ng/g. The overall recoveries range from 78% to 101% with RSD values between 1.8 and 7.2%.

Analysis of TCM injections using the Agilent 1290 Infinity LC system

The Dynamic MRM (DMRM) algorithm in the Agilent 6400 Series Triple Quadrupole LC/MS system automatically constructs DMRM timetables for multiple analytes throughout the LC/MS analysis based on the retention time window for each analyte. It allows the instrument to acquire MRM data only during a stated retention time window, thus reducing the number of concurrent ion transitions. In this study, 194 MRM transitions from a digest of complex protein standard was analyzed using DMRM and MRM modes in the Agilent 6410 Triple Quadrupole LC/MS system. Improved sensitivity, better peak symmetry, and up to ten-fold increase in the signal-to-noise (S/N) ratio are achieved using DMRM.

A fast multielement simulated distillation method (SimDis) based on Low Thermal Mass (LTM) column technology is described.

This application note describes a method for determining the boiling point range of biodiesel (B100 and B20) using the Agilent 7890A Series GC systems.

This Application Note describes how to use the Agilent 7700x ICP-MS with ISIS Discrete Sampling to perform high-throughput analysis of high-matrix samples. Using ISIS-DS, difficult, high-matrix analyses can be performed in a fraction of the time typically required, with no compromise in analytical performance.

A method for the detection and quantification of 2,4,6-Trichloroanisole in wine at a concentration as low as 1 ppt was developed using the Agilent 7890A/7000 Series Triple Quadrupole GC/MS with a Pressure Controlled Tee configuration.

The performance of sequential stir bar sorptive extraction (sequential SBSE) in combi- nation with thermal desorption (TD)-GC/MS for screening of pesticide residues in water was described.

This application note demonstrates a single quad GC/MS analysis of the GC/MS amenable listed allergens using the unique selectivity of an Agilent J&W DB-XLB capillary GC column

This application note describes a rapid method for the quantification of total testosterone in serum using the Agilent 1200 Series Rapid Resolution LC (RRLC) system with the Agilent 6430 Triple Quadrupole LC/MS in atmospheric pressure chemical ionization (APCI) mode. A two dimensional LC approach was used to minimize matrix interferences and ion suppression, while reducing the time required for analysis. Excellent linearity of quantification (R2 > 0.99) was obtained in the range of 5 to 2000 ng/dL, with analysis times of only 4.5 minutes, and day-to-day precision was below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were 2 ng/dL and 5 ng/dL respectively, in 5% bovine serum albumin.

This application brief describes a highly repeatable method for the quantification of estradiol in human plasma using the Agilent 7000 Triple Quadrupole GC/MS. The method provides an LOD of 1 pg/mL (2 fg on-column) and RSDs for quantification of <15% in the 1 to 600 pg/mL concentration range.