Agilent Manuals

This document describes a method for monitoring antibody charge variants using Agilent 3100 OFFGEL fractionation and high sensitivity protein detection with the Agilent 2100 Bioanalyzer. This method can be used for quality control in the production of monoclonal antibodies in the biopharmaceutical industry.

This paper describes a method for analyzing gold by atomic absorption. The method involves extracting gold with aqua regia and then analyzing the gold with flame atomic absorption spectrometry.

This application note describes the method for measuring chromium in environmental waters by atomic absorption spectrometry using a Zeeman corrected graphite tube atomizer. This method does not require the formation of a metal complex or extraction into an organic solvent for preconcentration, but directly injects the water sample into the graphite tube, which can reduce the possibility of sample preparation and contamination.

Various modifiers were investigated regarding their influence on the characteristic mass and memory effect for boron determination by graphite furnace spectrometry. The best results were obtained using a zirconium nitrate-nickel nitrate mixed modifier, which resulted in high sensitivity (characteristic mass 200 pg), negligible memory effect, and increased tube lifetime (about 200 atomization cycles at 2800 °C). The method was successfully applied to determine boron in river water, drinking water, and sewage.

This application note describes a method for determination of rhodium in catalyst oil and aqueous samples using Agilent 710 ICP-OES. The oil catalyst samples were prepared by microwave digestion and the aqueous samples were digested in nitric acid solution. The method provides good sensitivity and reproducibility and is suitable for determination of rhodium concentrations as low as 100 μg/L.

This document describes the method of analyzing Gabapentin in postmortem specimens using the Varian 1200L triple quadrupole LC/MS/MS. Gabapentin, an analog of the neurotransmitter GABA, is used to treat epilepsy. It also has many controversial off-label uses, such as in the treatment of bipolar disorder, social anxiety disorder, obsessive-compulsive disorder, and insomnia. The LC/MS/MS technique allows for a simple, cost-effective clean-up of the samples and provides accurate and sensitive results.

This document introduces an antibiotic called Lasalocid, which belongs to a group of antibiotics known as ionophores and can be used in the prophylaxis and treatment of coccidiosis and leukocytozoonosis in poultry. The document focuses on the ability of the Varian 320-MS Triple Quadrupole Mass Spectrometer to detect Lasalocid in animal feed.

This Application Note describes a method for establishing the dynamic range of the Varian 1200L using TX1360 and its major OH-Metabolite as the model compounds.

This application note describes a fast and simple method for removing rhodium using StratoSpheres™ SPE columns. StratoSpheres™ SPE columns are self-indicating SPE columns developed by Polymer Laboratories that change color in the presence of certain metal ions, informing the user when the column is nearing its capacity.

Synthesis of HIV-1 gp41 using AmphiSpheresTM Resin

This application note describes a method for synthesizing Leu-enkephalin using AmphiSpheresTM resin.

The document introduces the process of synthesizing ampr1 primer using StratoSpheres DNA dG support.

This application note describes a method for investigating the molecular weight distributions of diene rubber copolymers using an Agilent GPC system.

This white paper describes the synthesis of d(T)50 using Varian StratoSpheres DNA support.