Agilent Manuals

This paper describes the analysis of drugs of abuse using capillary electrophoresis coupled with electrospray ionization mass spectrometry (CE-ESI-MS). The unique identification of drugs was achieved by observing protonated molecular ions and characteristic fragment ions. The experimental results demonstrate the feasibility of the method, but additional method development and/or validation may be required for routine use.

The study proposes an optimized method for the analysis of tropane alkaloids by CE-ESI-MS. The results show that the method has high selectivity and sensitivity and is suitable for the quantitative analysis of tropane alkaloids in biological samples.

This application note describes a method for quantifying three alkaloids in the stem of various mahonia species: berberine, palmatine, and jatrorrhizine. The method uses capillary electrophoresis (CE), which is particularly suited to the analysis of plant extracts because of its ability to deal with complex or “dirty” sample matrices.

This paper compares the Agilent J&W DB-5ms Ultra Inert column with similar versions from four other column manufacturers with respect to their chromatographic behaviors for active analytes. Superior results were observed on the DB-5ms columns for basic compounds. Chromatograms depicting peak shape characteristics are presented to show how exceptional inertness leads to a significant reduction in tailing of active basic compounds for more accurate, reliable analytical results. Excellent chromato- graphic performance was obtained for a diverse group of compounds that included chlorophenols, organophosphorus pesticides and aromatic positional isomers demon- strating the applicability of the DB-5ms column for a wide range of applications.

This document describes the integration of Agilent ChemStation B.04.01 software with Agilent OpenLAB ECM software to meet the 21 CFR part 11 requirements

This document describes how to use Agilent ZORBAX Eclipse Plus C18 5µm, 3.5µm and 1.8µm columns for simvastatin analysis

This article describes the capillary zone electrophoresis analysis of neuropeptides. The method allows for rapid, sensitive, and simultaneous identification and quantification of diastereomeric peptides.

This document introduces the application of capillary electrophoresis in peptide analysis, including protein identification, peptide mapping, and purity checking of synthetic peptides. The authors describe the methodological aspects of peptide analysis, including the optimization of protein digestion buffers, electrophoretic conditions, reproducibility, detection sensitivity, and spectral analysis.

Lipidomics discovery profiling and targeted LC/MS analysis in 3T3-L1 differentiating adipocytes

This paper introduces a method for optimizing the separation of organic acids in fruit juice using low-pH ZORBAX Stable-Bond SB-Aq columns. The use of short columns, little organic solvent, and low-pH mobile phase enables rapid separation of organic acids in fruit juice.

This note introduces the methods for chiral separations by CE, including theory, method development strategies, experimental procedures etc.

This system addresses the challenges faced by laboratories performing toxicology screens on forensic samples by combining fast GC to reduce run time, simultaneous collection of scan, SIM, and NPD data in one shorter run, backflushing to prevent detector fouling, and Deconvolution Reporting Software (DRS) to simplify data interpretation. The system is able to find all the molecules detected by the conventional method in significantly less time.

This article describes the quantification of potential genotoxic impurity D in Atenolol using LC/ESI/MS/MS method with Agilent 1200 Series RRLC and 6410B Triple Quadrupole LC/MS instruments. The developed method is simple, shorter in runtime, with better resolution and higher sensitivity than the existing European and United States Pharmacopeia methods. Agilent's MassHunter Optimization software was used for quick optimization of MS parameters. By connecting the Agilent 6410B Triple Quadrupole (QQQ) LC/MS detector to an Agilent 1200 Series RRLC system, the quantification range can be effectively reduced further down to 5 ppb with a linearity coefficient greater than 0.9992.

Quantitative proteomics using multiple reaction monitoring (MRM) has emerged as an important methodology for biomarker validation. Multiple-reaction monitoring on a triple quadruple (QQQ) mass spectrometer provides superior sensitivity and selec- tivity for targeted peptides in a complex sample. MRM also offers high precision in quantitation and a fast scan speed, which makes it an ideal technology for validating biomarkers in a high-throughput fashion. This application note demonstrates the entire workflow for biomarker validation offered by Agilent Technologies using multiple reaction monitoring together with statistical analysis software. Thousands of peptide transitions were generated using Agilent Q-TOF and Spectrum Mill MRM selector. The transition list was imported into MassHunter acquisition software equipped with dynamic MRM functionality and monitored in a single LC/MS run. Finally, MRM quantitation results were analyzed using Mass Profiler Pro for principle component analysis (PCA), hierarchical clustering and ANOVA analysis.

This application note demonstrates the performance of the Agilent 1290 Infinity LC System coupled to an Agilent 6460A Triple Quadrupole LC/MS for the evaluation of metabolic stability using the cassette analysis approach combined with fast polarity switching.