Agilent Manuals

Agilent Low-Level Detection of Tylosin Using the 320-MS Triple Quadrupole Mass Spectrometer

This paper introduces a method for detecting low levels of tylosin using a 320-MS triple quadrupole mass spectrometer. This method can accurately detect and quantify tylosin over a wide concentration range and below EU required tolerance limits.

Agilent Simultaneous Detection of Steroid Amine Opiate Glucuronidation with a Single Rapid LC/MS/MS Method

The document provides basic knowledge about glucuronidation, including the enzyme family, gene differentiation, and substrates. Understanding UGTs and their substrates is becoming increasingly important from an epidemiological and pharmacological perspective.

Agilent Fatty Acids APPLICATION NOTE

Fatty Acids Application 2328 LC uses HPLC technology to analyze Pursuit™ 5µ C8, 150 x 4.6mm column and uses MeCN + 0.1% HCOOH and Water + 0.1% HCOOH as mobile phase, flow rate is 1.5 mL/min, detection wavelength is 205 nm, peaks are linolenic acid, linoleic acid and oleic acid.

Agilent beta-blockers APPLICATION NOTE

This is an application note about ß-Blockers Application 2326 LC technology

Agilent Vitamin B6 complex Application Note

This document describes the separation method of Vitamin B6 complex on Pursuit Diphenyl column. The mobile phase consists of 5 mM ammonium formate and CH3CN in a ratio of 95:5. The flow rate is 0.5 mL/min and detection is performed at 254 nm. This method is suitable for the separation and detection of pyridoxamine, pyridoxal, and pyridoxine.

Agilent Artificial Sweeteners Application Note

This document describes a method for separating artificial sweeteners on an Agilent Pursuit Diphenyl 5µ column.

Agilent Sorbic acid salicylic acid Application Note

This document describes the application of Sorbic acid and Salicylic acid on a Polaris Amide-C18 column, including the column dimensions, mobile phase composition, flow rate, temperature, and peak identification.

Agilent St. John's Wort APPLICATION NOTE

This document is an application note for St. John’s Wort Application 2219 LC Column, which introduces the features and usage of the product

Agilent Separation of anti-inflammatory/analgesic drugs

This application note describes the use of the Polaris C18-A column to separate anti-inflammatory/analgesic drugs. The column can be used in ChromSep or Conventional mode, and a gradient solvent system of 45%-80% B is used with a flow rate of 1.0 ml/min. Detection is at 220 nm.

Agilent Reverse-phase Peptide Standards: Dependence of Peptide separation on side chains Application Note

This application note describes the method of using Polaris C18-A column to separate peptide standards under various solvent conditions. The method shows the retention time and selectivity of peptide standards with different side chains, which is useful for analyzing different peptides and protein samples.

Agilent Goldenseal APPLICATION NOTE

This application note introduces an analytical method for the detection of hydrastine and berberine on Goldenseal using the Polaris 5µ C18-A, 150 x 4.6mm LC Column.

Agilent Sulfa drugs APPLICATION NOTE

This document provides a method for separating sulfonamide drugs using a Polaris 5µ C18-A, 150 x 4.6mm Part Nr.: A2000150X046 column

Agilent Analgesics APPLICATION NOTE(1)

This application note describes a procedure for the separation of four common analgesics by high performance liquid chromatography (HPLC) using a Polaris 5µ C8-Ether column. A 0.1% formic acid solution was used as the mobile phase. The four analgesics can be clearly seen in the chromatogram.

Agilent Insulin APPLICATION NOTE

This document is about the use of Insulin Application 2004 LC Column

Agilent Catechins Application Note

This document describes the LC column and gradient elution method for Catechins Application 1999, and provides the names and relative peak areas of four peaks for peak identification.