Key facts
- Brand
- RayBiotech
- Model
- CCL4 IQELISA Kit
- Category
- Health Care
- Industry
- Health
- Documents
- 3
- Languages
- EN
- Last added
- 2023-10-19
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RayBiotech MIP-1 beta/CCL4 IQELISA Kit User Manual
This document provides detailed instructions for using the RayBio® Mouse MIP-1 beta/CCL4 IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. It includes reagent preparation, assay procedure steps, and data calculation methods using Ct values. The kit is designed for use with real-time PCR instruments and requires specific materials and storage conditions for optimal performance.
The manual covers storage guidelines, reagent dilutions, standard preparation, and troubleshooting. It also explains how to calculate results using log-log graphs or linear regression, with formulas for determining unknown sample concentrations based on calibration curves.
RayBiotech CCL4 IQELISA Kit specifications
| Target Analyte | Human MIP-1 beta |
|---|---|
| Sample Types | serum, plasma, and cell culture supernatants |
| Plate Type | 96-well PCR plate |
| Minimum Quantifiable Dose | 0.97 pg/ml |
| Serum Spike Recovery | 86% (range 80% to 91%) |
| Intraplate CV | below 10% |
| Interplate CV | below 15% |
| Storage Temperature | 2° to 8°C |
Source: RayBiotech Mouse MIP-1 beta/CCL4 IQELISA Kit User Manual
All RayBiotech CCL4 IQELISA Kit documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the minimum quantifiable dose of MIP-1 beta in this kit?
The minimum quantifiable dose is typically 0.97 pg/ml.
What samples can be used to measure Human MIP-1 beta?
The assay is designed for the quantitative measurement of Human MIP-1 beta in serum, plasma, and cell culture supernatants.
What is the minimum quantifiable dose of MIP-1 beta?
The minimum quantifiable dose is typically 0.97 pg/ml.
From: RayBiotech Mouse MIP-1 beta/CCL4 IQELISA Kit User Manual
What sample types can be tested with this kit?
The kit is designed for serum, plasma, and cell culture supernatants.
From: RayBiotech Mouse MIP-1 beta/CCL4 IQELISA Kit User Manual
What is the minimum quantifiable dose of MIP-1 beta in this kit?
The minimum quantifiable dose is typically 0.97 pg/ml.
From: RayBiotech Mouse MIP-1 beta/CCL4 IQELISA Kit User Manual
What is the recovery rate for serum spike tests?
The recovery rate is 86%, with a range from 80% to 91%.
From: RayBiotech Mouse MIP-1 beta/CCL4 IQELISA Kit User Manual