This document is the user manual of RayBio MIP-1 beta/CCL4 IQELISA Kit
This document provides detailed instructions for using the RayBio® Mouse MIP-1 beta/CCL4 IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. It includes reagent preparation, assay procedure steps, and data calculation methods using Ct values. The kit is designed for use with real-time PCR instruments and requires specific materials and storage conditions for optimal performance.
The manual covers storage guidelines, reagent dilutions, standard preparation, and troubleshooting. It also explains how to calculate results using log-log graphs or linear regression, with formulas for determining unknown sample concentrations based on calibration curves.
| Minimum Quantifiable Dose | 0.97 pg/ml |
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The minimum quantifiable dose is typically 0.97 pg/ml.
The assay is designed for the quantitative measurement of Human MIP-1 beta in serum, plasma, and cell culture supernatants.
The minimum quantifiable dose of MIP-1 beta is typically 0.97 pg/ml.
The cycling protocol requires a 3 minute activation at 95°C, followed by 29 repeats of 10 seconds at 95°C and 25 seconds at 62°C.