RayBiotech Mouse IL-1 alpha IQELISA Kit Manuals

ManualLib has 2 documents for the RayBiotech Mouse IL-1 alpha IQELISA Kit (Health Care): 2 user manuals. Every file can be read online or downloaded as a PDF for free.

User Manual 2

Key facts

Model
Mouse IL-1 alpha IQELISA Kit
Category
Health Care
Documents
2
Languages
EN
Last added
2023-10-19

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RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual

RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual

User Manual 11 pages · 153 KB · EN

This document provides step-by-step instructions for using the RayBio® Mouse IL-1 alpha IQELISA Kit, including reagent preparation, assay procedure, and data calculation. The kit combines ELISA specificity with qPCR sensitivity for accurate quantification. It requires a real-time PCR instrument and standard lab equipment. Results are calculated using Ct values and a standard curve on log-log graph paper or software.

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RayBiotech Mouse IL-1 alpha IQELISA Kit specifications

Minimum Quantifiable Dose0.73 pg/ml
Recovery Rate121% (range 103% to 134%)
Inter-Plate CVBelow 15%

Source: RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual

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User Manual 2

Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.

Questions answered in these manuals

What is the minimum quantifiable dose of IL-1 alpha in this kit?

The minimum quantifiable dose is typically 0.73 pg/ml.

From: RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual

What is the recovery rate for this assay?

The recovery rate is 121%, with a range from 103% to 134%.

From: RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual

How should the standard recombinant protein be stored after reconstitution?

It should be stored at -20°C or -80°C, with -80°C being recommended.

From: RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual

What is the suggested dilution factor for normal serum or plasma samples?

The suggested dilution is 2 to 20 fold.

From: RayBiotech Mouse IL-1 alpha IQELISA Kit User Manual