RayBio® Mouse MIP-1 beta/CCL4 IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human MIP-1 beta in serum, plasma, and cell culture supernatants.
The RayBiotech Mouse MIP-1 beta/CCL4 IQELISA Kit is a modified ELISA assay with a high-sensitivity qPCR readout designed for the quantitative measurement of Human MIP-1 beta. The kit includes a 96-well PCR plate coated with anti-Human MIP-1 beta, various reagents including wash buffers, standards, and detection affinity molecules, and requires a real-time PCR instrument for analysis.
The assay procedure involves binding the target antigen to the coated plate, followed by a series of washes and incubations with detection reagents. The final step involves adding primers and a PCR master mix to the wells, which are then analyzed using a real-time PCR instrument with specific cycling parameters to generate Ct values for concentration calculation.
| Target Analyte | Human MIP-1 beta |
|---|---|
| Sample Types | serum, plasma, and cell culture supernatants |
| Plate Type | 96-well PCR plate |
| Minimum Quantifiable Dose | 0.97 pg/ml |
| Serum Spike Recovery | 86% (range 80% to 91%) |
| Intraplate CV | below 10% |
| Interplate CV | below 15% |
| Storage Temperature | 2° to 8°C |
The minimum quantifiable dose is typically 0.97 pg/ml.
The kit is designed for serum, plasma, and cell culture supernatants.
The kit may be stored for up to 6 months at 2° to 8°C from the date of shipment.
The standard should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution.