Key facts
- Brand
- RayBiotech
- Model
- Mouse IL-33 IQELISA Kit
- Category
- Health Care
- Documents
- 2
- Languages
- EN
- Last added
- 2023-10-19
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RayBiotech Mouse IL-33 IQELISA Kit User Manual
This document provides step-by-step instructions for using the RayBio® Mouse IL-33 IQELISA Kit, including reagent preparation, assay procedure, and result calculation. The kit combines ELISA specificity with qPCR sensitivity for quantifying Mouse IL-33 in serum, plasma, and cell culture supernatants.
The assay uses a 96-well PCR plate coated with anti-Mouse IL-33 antibody. Samples and standards are added, followed by detection reagents and PCR master mix. Data is collected via real-time PCR, and concentrations are calculated from Cq values using a log-scale graph or linear regression.
RayBiotech Mouse IL-33 IQELISA Kit specifications
| Catalog Number | IQM-IL33 |
|---|---|
| PCR Instrument Requirement | Real-time PCR instrument (Bio-Rad recommended) |
| Detection Wavelength | FITC compatible wavelength |
All RayBiotech Mouse IL-33 IQELISA Kit documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the detection method used in this assay?
It combines the specificity of ELISA with the sensitivity of real-time PCR, using qPCR to measure IL-33 concentration.
What is the PCR plate type used?
A 0.2 mL, non-skirted 96-well PCR plate (ThermoFisher, cat. no. AB0600).
What sample types can be tested with this kit?
Serum, plasma, and cell culture supernatants.
What is the catalog number for the Mouse IL-33 IQELISA Kit?
The catalog number is IQM-IL33.