Key facts
- Brand
- RayBiotech
- Model
- Mouse IL-1 beta IQELISA Kit
- Category
- Health Care
- Documents
- 2
- Languages
- EN
- Last added
- 2023-10-19
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RayBiotech Mouse IL-1 beta IQELISA Kit User Manual
This document provides step-by-step instructions for using the RayBio® Mouse IL-1 beta IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. It includes reagent preparation, assay procedure, and data calculation using Ct values. The kit requires a real-time PCR instrument and specific pipettes. Storage and handling instructions are provided to ensure accuracy and stability of results.
The assay uses a 96-well PCR plate coated with anti-Mouse IL-1b antibody. Samples and standards are added, followed by detection reagents and PCR master mix. Data is collected via qPCR, and concentrations are calculated using standard curves or linear regression.
RayBiotech Mouse IL-1 beta IQELISA Kit specifications
| Minimum Quantifiable Dose | 1.95 pg/ml |
|---|---|
| Recovery Range | 72% to 86% |
| Inter-Plate CV | Below 15% |
| PCR Plate Type | 0.2mL, non-skirted 96-well plate |
All RayBiotech Mouse IL-1 beta IQELISA Kit documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the minimum quantifiable dose of IL-1b in this kit?
The minimum quantifiable dose of IL-1b is typically 1.95 pg/ml.
What is the recovery rate for serum spike tests?
Serum spike tests show recovery is 80% with a range of 72% to 86%.
What is the minimum quantifiable dose of IL-1b?
The minimum quantifiable dose is typically 1.95 pg/ml.
What is the recommended storage temperature for the Standard?
The Standard should be stored at -20°C or -80°C after reconstitution.