RayBio® Mouse MIP-1 beta/CCL4 IQELISA Kit Protocol
This document provides detailed instructions for using the RayBio® Mouse MIP-1 beta/CCL4 IQELISA Kit, an innovative ELISA with qPCR readout for measuring Human MIP-1 beta. It includes reagent preparation, assay procedure, and data calculation steps. The kit requires a real-time PCR instrument and specific pipettes for accurate results.
The assay uses a 96-well PCR plate coated with anti-Human MIP-1 beta. Samples and standards are added, followed by detection reagents and PCR components. Data is collected via qPCR, and concentrations are calculated using Ct values and standard curves.
| Minimum Quantifiable Dose | 0.97 pg/ml |
|---|---|
| Recovery Rate | 86% |
| PCR Plate Type | 0.2mL, non-skirted 96-well plate |
The minimum quantifiable dose is typically 0.97 pg/ml.
The recovery rate is 86%, with a range from 80% to 91%.
The kit uses a 0.2mL, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600).