This manual provides instructions for the BD CBA Human Th1/Th2 Cytokine Kit II to quantitatively measure six cytokines in a single sample using flow cytometry.
The BD CBA Human Th1/Th2 Cytokine Kit II is designed to simultaneously detect Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor (TNF), and Interferon-gamma (IFN-gamma) protein levels in research samples such as tissue culture supernatants, EDTA plasma, and serum. The kit utilizes bead array technology where capture beads coated with specific antibodies form sandwich complexes with analytes, which are then detected using a flow cytometer.
| Sample types | Tissue culture supernatants, EDTA plasma, and serum |
|---|---|
| Test capacity per kit | 80 tests |
| Storage temperature | 2 to 8°C |
The theoretical limit of detection is comparable to conventional ELISA, though actual limits may vary slightly due to assay complexity.
No, the product is for Research Use Only and not for use in diagnostic or therapeutic procedures.
Stream-in-air instruments such as the BD FACStar Plus, BD Influx, and BD FACSVantage flow cytometers.