This manual provides training instructions for the BD FACS LSRFortessa flow cytometer, covering daily operations, maintenance, CS&T monitoring, and specific…
This document serves as a comprehensive training guide for the BD FACS LSRFortessa flow cytometer. It outlines the necessary equipment and reagents required for operation and details the standard operating procedures for daily startup and shutdown sequences to ensure instrument readiness.
The manual further covers monthly maintenance protocols, including cleaning cycles with FACSClean and FACSRinse solutions. It also explains how to perform CS&T (Cytometer Setup & Tracking) automated performance monitoring to adjust voltage settings and maintain data consistency. Additionally, it provides step-by-step instructions for creating application settings in BD FACSDiva software and conducting specific assays such as tricolor lymphocyte subset detection and DNA content analysis. This manual is written in Chinese.
The required centrifuge must be capable of 300g (equivalent to 1000-1500 rpm), have adjustable speed and time, and accommodate 12 x 75mm 5ml tubes.
After completing the startup steps, the instrument must be allowed to warm up for 10 minutes before use.
During the daily shutdown procedure, 500ml of sodium hypochlorite stock solution (final concentration approx. 10%) should be added to the waste bucket.