The document contains the use instructions for BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Kit II, including product name, model, application range and usage methods.
The BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Kit II is designed for the quantitative measurement of Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ) protein levels in a single sample. The kit utilizes six bead populations with distinct fluorescence intensities coated with capture antibodies specific to each cytokine, which are resolved in the red channel of a flow cytometer.
| Analytes Measured | IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ |
|---|---|
| Sample Types | Tissue culture supernatants, EDTA plasma, serum |
| Test Capacity per Vial | 80 tests (50 Samples and 2 standard curves) |
| Storage Temperature | 4°C |
The BD CBA is not recommended for use on stream-in-air instruments where signal intensities may be reduced, adversely affecting assay sensitivity.
The required sample volume is approximately one-sixth the quantity necessary for conventional ELISA assays due to the detection of six analytes in a single sample.