BD Phosflow Monocyte/NK Cell Activation Kit Instruction Manual

This instruction manual details the use of the BD Phosflow Monocyte/NK Cell Activation Kit for analyzing cell markers and intracellular phosphoproteins in…

Brand: BD Biosciences
Document type: User Manual
Language: English
Pages: 60
File format: PDF
File size: 1.3 MB
Published: 26 October, 2026
Updated: 26 October, 2026
MD5 checksum: FF88E0261E1A1312122ECBB5296B1929
PDF: BD Phosflow Monocyte/NK Cell Activation Kit Instruction Manual PDF

About this manual

The BD Phosflow Monocyte/NK Cell Activation Kit (Catalog No. 562089) provides reagents to simultaneously analyze multiple cell markers with one of several intracellular phosphoproteins for studying signaling events in human monocytes and NK cells. The kit enables flow cytometric analysis without prior purification of these populations from fresh whole blood.

What's inside

Purpose of the kit
Kit contents
Storage and safe handling
Workflow overview
Required materials
Preparation of cells and beads
Treating and staining the cells
Staining BD CompBeads
Cytometer procedures
Data analysis examples

Specifications

Catalog Number	562089BD
Sample Volume Required	200 µL of human peripheral blood
Reagent Volume per Sample	20 µL

Frequently asked questions

What is the purpose of this kit?

The kit provides reagents necessary to simultaneously analyze multiple cell markers with one of several intracellular phosphoproteins for studying monocyte and NK cell signaling events.

Is prior purification of cells required?

No, the kit enables flow cytometric phosphoprotein analysis without prior purification of the monocyte and NK cell populations from fresh whole blood.

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